Hypoxia-induced transcription factor signaling is essential for larval growth of the mosquito Aedes aegypti.

نویسندگان

  • Luca Valzania
  • Kerri L Coon
  • Kevin J Vogel
  • Mark R Brown
  • Michael R Strand
چکیده

Gut microbes positively affect the physiology of many animals, but the molecular mechanisms underlying these benefits remain poorly understood. We recently reported that bacteria-induced gut hypoxia functions as a signal for growth and molting of the mosquito Aedes aegypti In this study, we tested the hypothesis that transduction of a gut hypoxia signal requires hypoxia-induced transcription factors (HIFs). Expression studies showed that HIF-α was stabilized in larvae containing bacteria that induce gut hypoxia but was destabilized in larvae that exhibit normoxia. However, we could rescue growth of larvae exhibiting gut normoxia by treating them with a prolyl hydroxylase inhibitor, FG-4592, that stabilized HIF-α, and inhibit growth of larvae exhibiting gut hypoxia by treating them with an inhibitor, PX-478, that destabilized HIF-α. Using these tools, we determined that HIF signaling activated the insulin/insulin growth factor pathway plus select mitogen-activated kinases and inhibited the adenosine monophosphate-activated protein kinase pathway. HIF signaling was also required for growth of the larval midgut and storage of neutral lipids by the fat body. Altogether, our results indicate that gut hypoxia and HIF signaling activate multiple processes in A. aegypti larvae, with conserved functions in growth and metabolism.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Phylogenetic Analysis of Aedes aegypti Based on Mitochondrial ND4 Gene Sequences in Almadinah, Saudi Arabia

Background: Aedes aegypti is the main vector of the yellow fever and dengue virus. This mosquito has become the major indirect cause of morbidity and mortality of the human worldwide. Dengue virus activity has been reported recently in the western areas of Saudi Arabia. There is no vaccine for dengue virus until now, and the control of the disease depends on the control of the vector. Objectiv...

متن کامل

The Effectiveness of Trypsin Modulating Oostatic Factor (TMOF) and Combination of TMOF with Bacillus thuringiensis Against Aedes aegypti Larvae in the Laboratory

BACKGROUND Trypsin Modulating Oostatic Factor (TMOF) terminates trypsin biosynthesis in adult and larval mosquito gut. It will inhibit the growth and development of mosquito larvae feeding on it resulting in death by starvation. The objective of this study is to determine the effective dose of Pichia-TMOF and the combination of Pichia-TMOF with Bacillus thuringiensis (Bt) as larvicide on Ae. ae...

متن کامل

A laboratory study of cyromazine on Aedes aegypti and Culex quinquefasciatus and its activity on selected predators of mosquito larvae.

In a laboratory study, the insect growth regulator, cyromazine, exerted a high level of biological activity on Aedes aegypti and Culex quinquefasciatus treated in the 4th larval instar. At 1.5 and 1.0 ppm this IGR produced 97 and 99% inhibition of emergence in adult Ae. aegypti, respectively. In Cx. quinquefasciatus, there was 99% inhibition at 1 ppm and complete inhibition at 1.5 ppm. The over...

متن کامل

An inexpensive intervention for the control of larval Aedes aegypti assessed by an improved method of surveillance and analysis.

A sampling method coupled with statistical calibration factors was developed to accurately assess the numbers of larvae and pupae of Aedes aegypti in large water-storage containers of variable capacities and water levels. Aedes aegypti productivity in different types of breeding sites found in an urban study area in central Colombia was assessed and compared. In this study, water-storage tanks ...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:
  • Proceedings of the National Academy of Sciences of the United States of America

دوره 115 3  شماره 

صفحات  -

تاریخ انتشار 2018